3FVX
Human kynurenine aminotransferase I in complex with tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.881, 107.439, 81.686 |
| Unit cell angles | 90.00, 112.87, 90.00 |
Refinement procedure
| Resolution | 29.040 - 1.500 |
| R-factor | 0.17952 |
| Rwork | 0.178 |
| R-free | 0.20035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w7l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.285 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.076 | 0.590 |
| Number of reflections | 129473 | |
| <I/σ(I)> | 36.7 | 3.8 |
| Redundancy | 7.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 299 | 22@ PEG 4000, Sodium acetate 0.2 M, Tris 0.1 M, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
