3FVM

Crystal structure of Steptococcus suis mannonate dehydratase with metal Mn++

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-12-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	105.690, 105.690, 159.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.060 - 2.900
R-factor	0.2368
Rwork	0.234
R-free	0.28585
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tz9
RMSD bond length	0.014
RMSD bond angle	1.636
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	25673
<I/σ(I)>	5
Completeness [%]	94.0	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2M potassium/sodium tartrate, 0.1M sodium citrate, 1M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K