3FVA
NNQNTF segment from elk prion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.946496 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 18.061, 4.840, 21.362 |
| Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
| Resolution | 21.030 - 1.458 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.770 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
| Rmerge | 0.137 | 0.081 | 0.273 |
| Number of reflections | 750 | ||
| <I/σ(I)> | 7.197 | ||
| Completeness [%] | 98.7 | 100 | 100 |
| Redundancy | 5.4 | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M Ammonium Sulfate, 0.1M Tris pH 8.5, 25% PEG 3350, vapor diffusion, hanging drop, temperature 298K |
