3FV6
Crystal Structure of the CBS domains from the Bacillus subtilis CcpN repressor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2005-10-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.93300 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.751, 104.052, 99.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.560 - 1.950 |
R-factor | 0.20193 |
Rwork | 0.200 |
R-free | 0.27180 |
Structure solution method | SAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.058 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.087 | 0.191 |
Number of reflections | 19404 | |
<I/σ(I)> | 15.1 | 5.9 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 17% PEG 2000 MME, 0.1M HEPES, Protein 8mg/ml; Drops 1+1 microliter, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |