3FV6
Crystal Structure of the CBS domains from the Bacillus subtilis CcpN repressor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2005-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93300 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.751, 104.052, 99.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.560 - 1.950 |
| R-factor | 0.20193 |
| Rwork | 0.200 |
| R-free | 0.27180 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.058 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.087 | 0.191 |
| Number of reflections | 19404 | |
| <I/σ(I)> | 15.1 | 5.9 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 17% PEG 2000 MME, 0.1M HEPES, Protein 8mg/ml; Drops 1+1 microliter, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
