3FV5
Crystal Structure of E. coli Topoisomerase IV co-complexed with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.420, 72.840, 70.970 |
| Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
| Resolution | 19.750 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s14 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.038 |
| Completeness [%] | 81.4 |
| Redundancy | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 15% PEG 4000, 0.1M MES, pH=5.5, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
