3FV5
Crystal Structure of E. coli Topoisomerase IV co-complexed with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2003-04-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.420, 72.840, 70.970 |
Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
Resolution | 19.750 - 1.800 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s14 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.038 |
Completeness [%] | 81.4 |
Redundancy | 3.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 15% PEG 4000, 0.1M MES, pH=5.5, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |