3FUY

Structure from the mobile metagenome of Cole Harbour Salt Marsh: Integron Cassette Protein HFX_CASS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97929
Spacegroup name	P 32
Unit cell lengths	71.572, 71.572, 92.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.126 - 2.000
R-factor	0.1985
Rwork	0.195
R-free	0.23410
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.989
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.850	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.560
Total number of observations	202528
Number of reflections	35948
<I/σ(I)>	15.3	4.2
Completeness [%]	100.0	100
Redundancy	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.2M ammonium sulfate, 25% PEG 3350 pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.2M ammonium sulfate, 25% PEG 3350 pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K