3FUW
T. thermophilus 16S rRNA A1518 and A1519 methyltransferase (KsgA) in complex with 5'-methylthioadenosine in space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.417, 61.042, 82.531 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.630 - 1.560 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23500 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.341 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.600 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.063 | 0.480 |
Number of reflections | 38372 | |
<I/σ(I)> | 26.67 | 2.64 |
Completeness [%] | 97.0 | 94.5 |
Redundancy | 6.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 8.5 | 277 | 17% w/v polyethylene glycol 4000, 85 mM HEPES-NaOH (pH 8.5), 8.5% v/v isopropanol and 15% v/v anhydrous glycerol, microbatch technique under oil, temperature 277K |