3FUU
T. thermophilus 16S rRNA A1518 and A1519 methyltransferase (KsgA) in complex with Adenosine in space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.195, 61.263, 82.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.530 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.25800 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.414 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.570 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.077 | 0.410 |
Number of reflections | 41095 | |
<I/σ(I)> | 18.27 | 2.2 |
Completeness [%] | 98.3 | 99.6 |
Redundancy | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 7.5 | 277 | 50 mM magnesium chloride hexahydrate, 100 mM HEPES (pH 7.5) and 30% v/v polyethylene glycol monomethyl ether 550, microbatch technique under oil, temperature 277K |