3FUT
Apo-form of T. thermophilus 16S rRNA A1518 and A1519 methyltransferase (KsgA) in space group P21212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 137.046, 53.692, 69.354 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.040 - 1.520 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.24100 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.334 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.560 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.039 | 0.250 |
Number of reflections | 77536 | |
<I/σ(I)> | 34.67 | 4.27 |
Completeness [%] | 94.5 | 71.8 |
Redundancy | 5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 277 | 200 mM di-ammonium hydrogen phosphate and 20% w/v polyethylene glycole 3350, microbatch technique under oil, temperature 277K |