3FUA
L-FUCULOSE-1-PHOSPHATE ALDOLASE CRYSTAL FORM K
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994 |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.000, 183.000, 183.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.670 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.670 * | |
| Rmerge | 0.049 | |
| Total number of observations | 48988 * | |
| Number of reflections | 7316 * | |
| Completeness [%] | 95.0 * | 77 * |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.5 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.62 (M) | |
| 6 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 7 | 1 | reservoir | 4 (mM) | ||
| 8 | 1 | reservoir | beta-mercaptoethanol | 4 (mM) |
