3FU1
Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-15 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.638, 64.749, 65.041 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.280 - 1.900 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.187 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 0.512 |
Number of reflections | 17045 | |
<I/σ(I)> | 24.016 | 2.1 |
Completeness [%] | 97.1 | 78.8 |
Redundancy | 6.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 22.5% PEG 3350, 0.1M NA ACETATE, 0.04M ZN ACETATE, pH 5.0, vapor diffusion, sitting drop, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 22.5% PEG 3350, 0.1M NA ACETATE, 0.04M ZN ACETATE, pH 5.0, vapor diffusion, sitting drop, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 22.5% PEG 3350, 0.1M NA ACETATE, 0.04M ZN ACETATE, pH 5.0, vapor diffusion, sitting drop, temperature 298K |