3FTQ
Crystal structure of Septin 2 in complex with GppNHp and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.980, 118.440, 190.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.900 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qa5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.224 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.974 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.078 | 0.366 |
| Number of reflections | 33762 | |
| <I/σ(I)> | 4.81 | |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.2M Potassium Acetate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
