3FTL
NVGSNTY segment from Islet Amyloid Polypeptide (IAPP or Amylin), dehydrated crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.653, 4.824, 29.048 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 28.510 - 1.600 |
| R-factor | 0.127 |
| Rwork | 0.124 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.720 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.155 | 0.490 |
| Number of reflections | 1103 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 95.7 | 94 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 25% PEG 3350, vapor diffusion, hanging drop, temperature 298K |
