3FTH
NFLVHSS segment from Islet Amyloid Polypeptide (IAPP or Amylin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 9.725, 21.600, 26.086 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 16.600 - 1.840 |
| R-factor | 0.242 |
| Rwork | 0.238 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.556 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.189 | 0.460 |
| Number of reflections | 932 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 95.2 | 89.1 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 1.5M Ammonium Sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, vapor diffusion, hanging drop, temperature 298K |
