3FTF
Crystal structure of A. aeolicus KsgA in complex with RNA and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.130, 58.074, 68.981 |
| Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
| Resolution | 28.330 - 2.800 |
| R-factor | 0.2411 |
| Rwork | 0.239 |
| R-free | 0.28795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.880 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.144 | 0.397 |
| Number of reflections | 6848 | |
| <I/σ(I)> | 9 | 2.4 |
| Completeness [%] | 84.2 | 49.9 |
| Redundancy | 3.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 0.2 M KF, 40% MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KF | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 2 | KF | ||
| 4 | 1 | 2 | MPD |
