3FTE
Crystal structure of A. aeolicus KsgA in complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.173, 58.316, 91.118 |
| Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
| Resolution | 29.580 - 3.000 |
| R-factor | 0.2353 |
| Rwork | 0.233 |
| R-free | 0.28069 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.086 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.103 | 0.430 |
| Number of reflections | 7560 | |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 88.5 | 52.9 |
| Redundancy | 5.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 0.2 M NH4NO3, 40% MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4NO3 | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 2 | NH4NO3 | ||
| 4 | 1 | 2 | MPD |
