3FTC
Crystal structure of A. aeolicus KsgA at 1.72-Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.875, 53.577, 101.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.510 - 1.680 |
| R-factor | 0.2078 |
| Rwork | 0.206 |
| R-free | 0.24606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.063 | 0.500 |
| Number of reflections | 25134 | |
| <I/σ(I)> | 25.7 | 1.7 |
| Completeness [%] | 92.3 | 54.8 |
| Redundancy | 6.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 25% PEG 3000, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
