3FT7
Crystal structure of an extremely stable dimeric protein from sulfolobus islandicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-07 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 |
Unit cell lengths | 33.030, 33.030, 85.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 85.749 | 33.030 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.080 | 0.034 | 0.623 |
Total number of observations | 840 | 3846 | |
Number of reflections | 6218 | ||
<I/σ(I)> | 6.307 | 11.9 | 1.2 |
Completeness [%] | 99.6 | 98.2 | 99.7 |
Redundancy | 4.2 | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16-22% PEG 4000, 100mM HEPES pH 7.5 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |