3FT7

Crystal structure of an extremely stable dimeric protein from sulfolobus islandicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-07-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	33.030, 33.030, 85.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.030 - 2.000
R-factor	0.177
Rwork	0.171
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1myk 1par
RMSD bond length	0.016
RMSD bond angle	1.933
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.749	33.030	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.080	0.034	0.623
Total number of observations		840	3846
Number of reflections	6218
<I/σ(I)>	6.307	11.9	1.2
Completeness [%]	99.6	98.2	99.7
Redundancy	4.2	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	16-22% PEG 4000, 100mM HEPES pH 7.5 , VAPOR DIFFUSION, HANGING DROP, temperature 293K