3FSZ
Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.824, 86.959, 41.848 |
Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
Resolution | 21.440 - 2.000 |
R-factor | 0.18776 |
Rwork | 0.184 |
R-free | 0.25507 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.100 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.094 | 0.328 |
Number of reflections | 13807 | |
<I/σ(I)> | 18.7 | 5 |
Completeness [%] | 89.9 | 83.2 |
Redundancy | 6.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 10mM tri-sodium citrate, 33% PEG 6000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |