3FSS
Structure of the tandem PH domains of Rtt106
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-17 |
Wavelength(s) | 0.98089 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.409, 92.206, 34.273 |
Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
Resolution | 29.508 - 1.432 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.21020 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.508 | 1.463 |
High resolution limit [Å] | 1.432 | 1.432 |
Number of reflections | 45389 | |
Completeness [%] | 98.1 | 81 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 4% Tacsimate, 12% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |