3FSD

Crystal structure of NTF2-like protein of unknown function in nutrient uptake (YP_427473.1) from RHODOSPIRILLUM RUBRUM ATCC 11170 at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97867,0.97776,0.91162
Spacegroup name	P 42 21 2
Unit cell lengths	61.018, 61.018, 56.442
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.287 - 1.700
R-factor	0.175
Rwork	0.172
R-free	0.22100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.684
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.287	27.290	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.095	0.051	0.755
Total number of observations		1286	8443
Number of reflections	12272
<I/σ(I)>	15.8	34.6	2.4
Completeness [%]	100.0	98.2	100
Redundancy	9.4	7.4	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20.0% polyethylene glycol 3350, 0.2M magnesium chloride, ADDITIVE: 0.001M uridine 5'-monophosphate (UMP), NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K.