3FS3
Crystal structure of malaria parasite Nucleosome Assembly Protein (NAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.408, 38.117, 80.046 |
| Unit cell angles | 90.00, 107.87, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.218 |
| R-free | 0.28500 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.238 |
| Number of reflections | 12065 | |
| <I/σ(I)> | 26.1 | 4.98 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 20% PEG 3350, 0.2M magnesium chloride, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
