3FRV

Structure of Human CHMP3 (residues 1-150)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-11-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	111.484, 111.484, 30.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 3.700
R-factor	0.41
Rwork	0.435
R-free	0.37500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gd5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.830
High resolution limit [Å]	3.700	3.700
Rmerge	0.630	0.553
Number of reflections	2451
<I/σ(I)>	18.3	2.2
Completeness [%]	99.2	99.2
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	286	16% PEG 3350, 100 mM HEPES, 200mM Proline, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K