3FRS

Structure of human IST1(NTD) (residues 1-189)(p43212)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-09-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	57.180, 57.180, 157.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.610
R-factor	0.261
Rwork	0.259
R-free	0.29800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.177
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.610	5.620	2.610
Rmerge	0.079	0.051	0.459
Number of reflections	8387
<I/σ(I)>	13.47
Completeness [%]	97.7	93.6	88.3
Redundancy	3.8	3.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	296	10mM Tris-HCL, pH 8.0, 350mM NaCl, 1mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 296K
1	VAPOR DIFFUSION, SITTING DROP	7	296	10mM Tris-HCL, pH 8.0, 350mM NaCl, 1mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 296K