3FRQ

Structure of the macrolide biosensor protein, MphR(A), with erythromcyin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	44.294, 61.201, 134.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.270 - 1.760
R-factor	0.18303
Rwork	0.181
R-free	0.22130
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.554
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.270
High resolution limit [Å]	1.760	1.760
Number of reflections	35192
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	35-40% (W/V) PEG 3350, 0.2M MgCl2, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K