3FRO
Crystal structure of Pyrococcus abyssi glycogen synthase with open and closed conformations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.998, 139.913, 159.414 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.910 - 2.500 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25500 |
Starting model (for MR) | 2bis |
RMSD bond length | 0.005 |
RMSD bond angle | 0.825 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.078 | 0.410 |
Number of reflections | 58364 | |
<I/σ(I)> | 8.4 | 1.1 |
Completeness [%] | 99.7 | 97.6 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 26-30% MPD, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |