3FRM
The crystal structure of a functionally unknown conserved protein from Staphylococcus epidermidis ATCC 12228.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.711, 126.549, 112.807 |
| Unit cell angles | 90.00, 94.52, 90.00 |
Refinement procedure
| Resolution | 41.450 - 2.320 |
| R-factor | 0.20003 |
| Rwork | 0.197 |
| R-free | 0.26346 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.878 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.370 |
| High resolution limit [Å] | 2.320 | 2.330 |
| Rmerge | 0.115 | 0.732 |
| Number of reflections | 76418 | |
| <I/σ(I)> | 16.5 | 1.71 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.2M Li2SO4, 0.1M Acetate, 2.5M NaCl, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
