3FRI
Structure of the 16S rRNA methylase RmtB, I222
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2007-05-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.480, 94.460, 98.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.550 - 1.800 |
R-factor | 0.2125 |
Rwork | 0.212 |
R-free | 0.23290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b89 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.375 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.450 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 0.394 |
Number of reflections | 24613 | |
<I/σ(I)> | 17.33 | 3.21 |
Completeness [%] | 99.1 | 99.4 |
Redundancy | 3.58 | 3.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG 3350, 50mM Bis-Tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |