3FRH
Structure of the 16S rRNA methylase RmtB, P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.856, 48.304, 54.558 |
| Unit cell angles | 90.00, 115.05, 90.00 |
Refinement procedure
| Resolution | 25.400 - 1.200 |
| R-factor | 0.1388 |
| Rwork | 0.139 |
| R-free | 0.15930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b89 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.078 | 1.400 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.039 | 0.130 |
| Number of reflections | 71817 | |
| <I/σ(I)> | 14.56 | 7.73 |
| Completeness [%] | 95.6 | 94.3 |
| Redundancy | 2.2 | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG 3350, 50mM bis-tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
