3FQI
Crystal Structure of the Mouse Dom3Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.979, 88.379, 50.069 |
| Unit cell angles | 90.00, 114.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.013 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.077 | 0.061 | 0.190 |
| Number of reflections | 23658 | ||
| <I/σ(I)> | 17.139 | ||
| Completeness [%] | 98.3 | 94 | 99.8 |
| Redundancy | 3.1 | 2.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M Hepes (pH 7.5) and 20% (w/v) PEG 8000, vapor diffusion, sitting drop, temperature 295K |
