3FPX
Native fungus laccase from Trametes hirsuta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-11 |
| Detector | MAR |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.293, 76.345, 129.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.900 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.826 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.900 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.840 |
| Rmerge | 0.074 | 0.580 |
| Number of reflections | 46382 | |
| <I/σ(I)> | 19.8 | 2.7 |
| Completeness [%] | 94.7 | 81.3 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7 | 299 | 10% PEG 1000, 0.02M potassium phosphate buffer, pH7.0, HANGING DROP, temperature 299.0K |
