3FPB
The Structure of Sarcoplasmic Reticulum Ca2+-ATPase Bound To Cyclopiazonic acid with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.360, 69.870, 143.500 |
| Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
| Resolution | 24.942 - 2.550 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o9j |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.430 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 54351 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 284 | 8% PEG 3350, 21% GLYCEROL, 20mM MGCL2, 0.1mM EGTA, 20mM MES, pH 6.10, VAPOR DIFFUSION, HANGING DROP, temperature 284K |
