3FP5

Crystal structure of ACBP from Moniliophthora perniciosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-09-19
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	32.500, 41.024, 82.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.680 - 1.610
R-factor	0.188
Rwork	0.186
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fj9
RMSD bond length	0.007
RMSD bond angle	0.928
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.239	1.680
High resolution limit [Å]	1.606	1.590
Rmerge	0.051	0.368
Number of reflections	14876
<I/σ(I)>		2
Completeness [%]	97.5	84.1
Redundancy	13.1	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	291	100mM Mes pH 6.5, 25% PEG 550MME, 10mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K