3FP4

Crystal structure of Tom71 complexed with Ssa1 C-terminal fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2008-10-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	47.820, 116.025, 150.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.140
R-factor	0.21036
Rwork	0.208
R-free	0.26127
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fp3
RMSD bond length	0.023
RMSD bond angle	1.787
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.140	2.140
Number of reflections	39166
Completeness [%]	0.9	0.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG6K, 5% Ethylene Glyco, 0.15M NaCl, Tris 10mM, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K