3FP2
Crystal structure of Tom71 complexed with Hsp82 C-terminal fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2008-10-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.863, 116.288, 150.743 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.990 - 1.980 |
R-factor | 0.20107 |
Rwork | 0.199 |
R-free | 0.23645 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fp3 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.524 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 7.990 | |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 53827 | |
Completeness [%] | 1.0 | 0.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG6K, Ethylene Glyco 5%, NaCl 0.15M, Tris 10mM, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |