3FOZ

Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	89.240, 91.260, 152.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.24517
Rwork	0.244
R-free	0.26822
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d3q 1ttt
RMSD bond length	0.008
RMSD bond angle	1.279
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.290	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	43749
<I/σ(I)>	6.8	2.14
Completeness [%]	99.8	100
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 4K 15%, 0.2M CaCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	1	CaCl2
3	1	2	PEG 4000
4	1	2	CaCl2