3FOV
Crystal structure of protein RPA0323 of unknown function from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.341, 44.747, 32.661 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 26.270 - 1.880 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.488 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.300 | 1.940 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.067 | 0.445 |
| Number of reflections | 8519 | |
| <I/σ(I)> | 40.099 | 6.2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10.4 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294 | 20% PEG 3350, 0.2 M Magnesium nitrate, 0.3 M NSDB-256, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
