3FOP
A Triangular Cytochrome b562 Superstructure Mediated by Ni Coordination - Hexagonal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97607 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 104.897, 104.897, 107.925 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.910 - 3.000 |
| R-factor | 0.225 |
| Rwork | 0.221 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qla |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.441 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.833 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.151 | 0.600 |
| Total number of observations | 17168 | |
| Number of reflections | 7479 | |
| <I/σ(I)> | 4.7 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 25% PEG2000, 0.2 M MgCl2, 0.1 M TRIS, 3.3 mM NiCl2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
