3FOL
Crystal structure of the Class I MHC Molecule H-2Kwm7 with a Single Self Peptide VNDIFERI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.393, 66.630, 56.211 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.500 |
| R-factor | 0.196 |
| Rwork | 0.192 |
| R-free | 0.26100 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.601 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.068 | 0.035 | 0.819 |
| Number of reflections | 18990 | ||
| <I/σ(I)> | 19.169 | ||
| Completeness [%] | 97.4 | 99.7 | 76.4 |
| Redundancy | 7.1 | 7.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG 3350, 200 mM ammonium sulfate, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
