3FOC

Tryptophanyl-tRNA synthetase from Giardia lamblia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97945
Spacegroup name	P 21 21 2
Unit cell lengths	106.714, 140.147, 90.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.090
R-factor	0.2
Rwork	0.199
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ip1
RMSD bond length	0.007
RMSD bond angle	1.003
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.180
High resolution limit [Å]	2.090	4.520	2.090
Rmerge	0.089	0.051	0.610
Number of reflections	77780
<I/σ(I)>	20.921
Completeness [%]	97.3	99.9	82
Redundancy	6.9	7.2	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.2	298	2.7 M (NH4)2SO4, 0.1 M Citric acid pH 5.2, 5mM DTT, VAPOR DIFFUSION, temperature 298K