3FNR

CRYSTAL STRUCTURE OF PUTATIVE ARGINYL T-RNA SYNTHETASE FROM Campylobacter jejuni;

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-07
Detector	ADSC QUANTUM 315
Spacegroup name	P 61 2 2
Unit cell lengths	106.595, 106.595, 210.017
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.2151
Rwork	0.214
R-free	0.25785
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.116
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.081	0.890
Number of reflections	36585
<I/σ(I)>	5.7	1.9
Completeness [%]	100.0	100
Redundancy	9.3	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	100 MM BIS-TRIS, 200 MM AMMONIUM SULFATE, 25% (V/V) PEG3350, 10% GLYCEROL, PH 5.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K