3FNJ
Crystal structure of the full-length lp_1913 protein from Lactobacillus plantarum, Northeast Structural Genomics Consortium Target LpR140
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97905 |
Spacegroup name | P 43 |
Unit cell lengths | 119.365, 119.365, 67.791 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.700 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.27600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.085 | 0.421 |
Number of reflections | 51464 | |
<I/σ(I)> | 26.7 | 4.4 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.5 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 7 | 293 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 100 mM Bis-Tris Propane (pH 7.0) and 1880 mM sodium thiosulfate. , Microbatch under oil, temperature 293K |