3FNC
Crystal structure of a putative acetyltransferase from Listeria innocua
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-30 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97962, 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.974, 61.030, 75.132 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 28.270 - 1.750 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.073 | 0.268 |
| Number of reflections | 35751 | |
| <I/σ(I)> | 27.534 | |
| Completeness [%] | 97.7 | 81.1 |
| Redundancy | 4.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.4M Na Malonate, DMSO, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
