3FN4
Apo-form of NAD-dependent formate dehydrogenase from bacterium Moraxella sp.C-1 in closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.660, 66.089, 75.551 |
| Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
| Resolution | 39.100 - 1.960 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsd |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.820 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.050 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.122 | 0.476 |
| Number of reflections | 27648 | |
| <I/σ(I)> | 2.4 | 1.3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Protein solution (2mcl): 10.5 mg/ml FDH, 0.1M Na2HPO4 pH 7.0, Reservoir solution (2mcl): 0.1M HEPES pH 7.5, 2.2M Ammonium Sulfate, 2% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
