3FN2
Crystal structure of a putative sensor histidine kinase domain from Clostridium symbiosum ATCC 14940
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-09 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97945, 0.97921 |
Spacegroup name | H 3 |
Unit cell lengths | 107.252, 107.252, 49.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.19 |
Rwork | 0.187 |
R-free | 0.25100 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.475 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.091 | 0.486 |
Number of reflections | 16144 | |
<I/σ(I)> | 30.418 | |
Completeness [%] | 95.7 | 73.6 |
Redundancy | 5.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 296 | 0.1M Acetate pH 4.5, 0.8M NaH2PO4, 1.4M K2HPO3, VAPOR DIFFUSION, SITTING DROP, temperature 296K |