3FMX
Crystal structure of Tartrate dehydrogenase from Pseudomonas putida complexed with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-26 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 182.906, 182.906, 66.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| R-factor | 0.26191 |
| Rwork | 0.260 |
| R-free | 0.29684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3flk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.383 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 14251 | |
| Completeness [%] | 84.7 | 80.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.65 M ammonium sulfate, 0.2 M ammonium tartrate, 0.01 M DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
