3FMU
Crystal Structure Analysis of Fungal Versatile Peroxidase from Pleurotus eryngii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.891976 |
| Spacegroup name | P 43 |
| Unit cell lengths | 63.199, 63.199, 99.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.688 - 1.040 |
| R-factor | 0.11151 |
| Rwork | 0.111 |
| R-free | 0.12301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fkg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.150 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.108 | 0.299 |
| Number of reflections | 186809 | |
| <I/σ(I)> | 11.95 | 4.57 |
| Completeness [%] | 97.6 | 91.8 |
| Redundancy | 3.98 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 9.0mg/ml protein in 10mM Na-tartrate pH 5.5, 14% PEG 8000, 100mM Zn-acetate, 100mM Na-cacodylate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
