3FMT
Crystal structure of SeqA bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 |
Unit cell lengths | 121.839, 121.839, 279.133 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.172 - 2.983 |
R-factor | 0.2194 |
Rwork | 0.218 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1LRR and 1XRX |
RMSD bond length | 0.004 |
RMSD bond angle | 0.877 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.170 | 3.110 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.088 | 0.527 |
Number of reflections | 30745 | |
<I/σ(I)> | 13.5 | 1.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 18% MPD, 0.4 M ammonium acetate, 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | ammonium acetate | ||
3 | 1 | 1 | sodium citrate | ||
4 | 1 | 2 | MPD | ||
5 | 1 | 2 | ammonium acetate | ||
6 | 1 | 2 | sodium citrate |