3FMO
Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.484, 115.392, 143.461 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.790 - 2.510 |
R-factor | 0.20102 |
Rwork | 0.199 |
R-free | 0.24199 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oit |
RMSD bond length | 0.014 |
RMSD bond angle | 1.492 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 33892 | |
<I/σ(I)> | 2.7 | |
Completeness [%] | 98.6 | 89.9 |
Redundancy | 13.8 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | sodium di-hydrogen phosphate, di-potassium hydrogen phosphate, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |