3FMB

Crystal structure of dimeric protein of unknown function and ferredoxin-like fold (YP_212648.1) from Bacteroides fragilis NCTC 9343 at 1.85 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97867
Spacegroup name	P 61 2 2
Unit cell lengths	72.310, 72.310, 233.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.399 - 1.850
R-factor	0.172
Rwork	0.170
R-free	0.20500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.468
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.399	29.399	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.090	0.012	0.012
Number of reflections	31933	5884	6033
<I/σ(I)>		39.3	1.7
Completeness [%]	99.7	99.5	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	NANODROP, 0.2M (NH4)2SO4, 20.0% PEG 3350, No Buffer pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K