3FMB
Crystal structure of dimeric protein of unknown function and ferredoxin-like fold (YP_212648.1) from Bacteroides fragilis NCTC 9343 at 1.85 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97867 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 72.310, 72.310, 233.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.399 - 1.850 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20500 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.468 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.399 | 29.399 | 1.920 |
High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
Rmerge | 0.090 | 0.012 | 0.012 |
Number of reflections | 31933 | 5884 | 6033 |
<I/σ(I)> | 39.3 | 1.7 | |
Completeness [%] | 99.7 | 99.5 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | NANODROP, 0.2M (NH4)2SO4, 20.0% PEG 3350, No Buffer pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |